ABSTRACT
Abstract The seeds of Plukenetia polyadenia have high levels of unsaturated fatty acids and are used as medicine and food for native people in the Peruvian and Brazilian Amazon. The objective of this study was to develop a method for vegetative propagation of Plukenetia polyadenia by rooting of cuttings. The experiment was laid out in a randomized complete block design with 12 treatments and 3 replications of 8 cuttings, in a 3 × 4 factorial arrangement. The factors were: 3 levels of leaf area (25, 50 and 75%) and 3 indole-3-butyric acid - IBA concentrations (9.84, 19.68 and 29.52mM) and a control without IBA. Data were submitted to analysis of variance and means were compared by Tukey test at 5% probability. Our results show that the use of cuttings with 50% of leaf area and treatment with 29.52mM of IBA induced high percentages of rooting (93%) and the best root formation. Vegetative propagation of Plukenetia polyadenia by cuttings will be used as a tool to conserve and propagate germplasm in breeding programs.
Resumo As sementes de Plukenetia polyadenia têm altos níveis de ácidos graxos insaturados e são utilizadas como medicamentos e alimentos para as pessoas nativas da Amazônia Peruana e Brasileira. O objetivo do trabalho foi desenvolver um método de propagação vegetativa de Plukenetia polyadenia por meio do enraizamento de estacas em câmeras de sub-irrigação. Foi utilizado um delineamento de blocos ao acaso com 12 tratamentos e 3 repetições de 8 estacas, e esquema fatorial 3 × 4. Os fatores foram: 3 níveis de área foliar (25, 50 e 75%) e 3 doses de ácido indol-3-butírico - AIB (9,84; 19,68 e 29,52mM) e um controle sem AIB. Os dados foram submetidos à análise de variância e as médias foram comparadas pelo teste de Tukey a 5% de probabilidade. A maior taxa de enraizamento de estacas (93%) foi obtida com 29,52mM de AIB como indutor hormonal e estacas com área foliar de 50%. A propagação vegetativa de Plukenetia polyadenia por estacas será usada como ferramenta para conservar e propagar germoplasma em programas de melhoramento.
Subject(s)
Plant Growth Regulators/pharmacology , Reproduction, Asexual , Euphorbiaceae/growth & development , Plant Breeding/methods , Indoles/pharmacology , Plant Leaves/anatomy & histologyABSTRACT
Mangrove forests continue to disappear all over the world due to a number of reasons. This is the study made to screen the anatomical and biochemical changes during the rooting process. Two important mangrove species have been examined based on their salt relationship natures: Avicennia officinalis subsp. australasica (salt excreting plant) and Excoecaria agallocha L (salt accumulating plant). The plants were treated with different root promoting hormones like IBA, IAA and NAA at different concentrations for three minutes each. After 45 days of the growth period root growth and anatomical changes were observed. The roots originated from the deeper zone of the secondary xylem after several anatomical variations in the hormone treated plants. This is the first trial which may help to determine the mass propagation of these commercial and medicinally important mangroves.